課程名稱︰蛋白質體學
課程性質︰選修
課程教師︰主授張英峰（此範圍為張英峰/鄭貽生老師共同授課）
開課學院：生命科學院
開課系所︰生命科學系
考試日期（年月日）︰2017/04/17
考試時限（分鐘）：120分鐘
考試範圍：
Introduction (張英峰)
From genome to proteome (鄭貽生)
Sample preparation (張英峰)
Protein separation and detection (張英峰)
Protein digestion and peptide separation (張英峰)
Mass spectrometry (張英峰)
試題 :
A. Please choose one right answer for each questions: (15%)
1. What is the maximum miss cleavage number for the following peptide
EKMSTELKEYRTVWYM if trypsin is used?
(1) 0; (2)1; (3)2; (4)3; (5)4
2. If m/z value 1334.5 was observed using MALDI-TOF, what is the actual mass
of the analyte?
(1) 1334.5; (2) 1335.5; (3) 1333.5; (4) 2669; (5) 667.7
3. Which of the following does not aim at sub-proteome analysis?
(1) Organelle isolation;
(2) Affinity purification of protein complex;
(3) Phosphopeptide enrichment;
(4) Total protein isolation and analysis
4. What feature of peptides is used for C4 Zip-tip to clean up proteins?
(1) charge; (2) mass; (3) hydrophobicity; (4) size
5. Which of the following does not have tandem mass spectrometer?
(1) Q-TOF; (2) TOF-TOF; (3) MALDI-TOF; (4) Triple-Q
B. Please link the two: (10%)
1. ESI a. y. b ions
2. MALDI b. zwitterion
3. Ampholyte c. mutiple charged ions
4. CID d. cy3, cy5
5. DIGE e. singly charged ions
1-c, 2-e, 3-b, 4-a, 5-d
C. Please define the following terms: (15%)
1. Edman degradation
2. Alpha-CHCA
3. ETD
4. CE-MS
5. Zwitterion
D. Please explain "Why is Proteomics necessary for scientific research?" (14%)
E. Please explain how NEphGE works in a proteomic study. (5%)
F. Please explain how MALDI works in a mass spectrometer. (5%)
G. Please write out the contribution of researchers John Fenn, Koichi Tanaka
and John Yates respectively. (5%)
H. Please describe how internal calibration is performed for MALDI-TOF
analysis? (5%)
I. Please describe how MudPIT works in a proteomic study. (10%)
J. Please describe and compare different protein staining methods. (6%)
K. What is the possible cleavage fragment for the peptide TGMHLETYKDERLSK
if trypsin, V8, and CNBr are used individually? (10%)
(PS. 本堂課無期末考，期末需繳交mini-review，期中考目的重在了解基本原理)